Introduction:

DNA Purification Kit is designed to extract and purify DNA from any agarose gel in either TAE or TBE buffer without phenol extraction or ethanol precipitation. The kit combines the convenience of spin-column technology with the selective binding properties of a uniquely designed silica membrane. Special buffers are optimized for efficient recovery of DNA and removal of contaminants. The Adsorption Column can purify the fragments ranging from 100bp to 10kb with over 80% of recovery efficiency from primers, nucleotides, polymerases, and salts, etc (The recovery rates of DNA fragments smaller than 100bp and larger than 100kb is 30-50%.

The Kit provide high yields of pure nucleic acids, for direct use in applications such as restriction enzyme digestion, PCR, sequencing, library screening, ligation and transformation, in vitro translation, and transformation of robust cells.

Important Notes:

1. Use fresh electrophoresis buffer on
2. Apply TAE electrophoresis buffer if there is high demand for following experiment
3. Shorten ultraviolet irradiation time as much as possible when cutting agarose
4. Increasing time of absorption and elution, volume of Buffer PN / Binding could improve recovery efficiency for <100bp and >10kb DNA fragment.
5. The purifying efficiency is related to starting DNA quantity and elution volume. Insufficient starting quantity or elution volume reduces the recovery efficiency significantly.
6. Unless otherwise specified, all centrifugation steps are centrifuged at room temperature using a benchtop centrifuge.
   

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   Note：
   1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
   2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
   3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.