Product Description:

α-GC (EC 3.2.1.20) is widely existed in animals, plants, microorganisms and cultured cells, which catalyzes the hydrolysis of α-glycosidic bonds between aryl or hydrocarbyl groups and glycosyl groups to form glucose, which is not only related to the relaxation or reinforcement of cell walls, but also closely related to cell recognition and the production of some signaling molecules.

α-GC decomposes p-nitrophenyl-α-D-glucopyranoside to form p-nitrophenol, which has a maximum absorption peak in 400 nm. The activity of α-GC is calculated by measuring the increasing rate of absorbance value.

Reagents and Equipment Required but Not Provided：

Spectrophotometer, desk centrifuge, water bath/constant temperature incubator, sonicator, transferpettor, mortar/homogenizer, 1 mL glass cuvette, ice and distilled water.

Components:

Extraction: Liquid 50 mL×1.Store at 2-8℃.

Reagent I: Powder×2. Store at -20℃. Add 10 mL distilled water to each bottle before use, fully dissolved. Unused reagents can be dispensed and stored at -20℃ for 4 weeks.. Avoid repeating freeze thaw cycles.

Reagent Ⅱ: Liquid 25 mL×1. Store at 2-8℃.

Reagent Ⅲ: Liquid 80 mL×1. Store at 2-8℃.

Standard: Liquid 1 mL×1. Store at 4℃. 5 μmol/mL p-nitrophenol solution.