Introduction :

This product is an efficient and convenient first-strand cDNA synthesis reagent. The Finepure First-strand cDNA Synthesis Mix contains Finepure Reverse Transcriptase and its reaction Buffer, Rnasin, dNTPs, Oligo(dT)20VN and random primers and other first-strand cDNAs All the components required for synthesis are ready for reverse transcription by adding RNA template and water.

The Finepure Reverse Transcriptase contained in this product is a new generation of reverse transcriptase, 50℃ reaction, strong thermal stability, high reverse transcription efficiency. dsDNase can effectively remove the residual genomic DNA in the RNA template, ensuring more reliable subsequent quantitative results, and qPCR primers do not need to be designed across introns. The cDNA obtained by reverse transcription of this product can be used for downstream qPCR detection.

Highlights:

1.         Simple and fast: genome removal and reverse transcription can be completed in one or two steps. Just add RNA template and water to perform reverse transcription reaction;

2.         Strong thermal stability: the reaction at 50℃ improves the reverse transcription efficiency of the complex secondary structure of the RNA template;

3.         High reverse transcription efficiency: Compared with similar products on the market, the reverse

dsDNase can effectively remove the residual genomic DNA in the RNA template, ensuring more reliable subsequent quantitative results, and qPCR primers do not need to be designed across introns. The cDNA obtained by reverse transcription of this product can be used for downstream qPCR detection.

Usage :

This product is suitable for the first-strand cDNA synthesis reaction of animal, plant and microbial RNA templates. The product obtained by reverse transcription of this product is compatible with downstream dye-based qPCR detection and probe-based qPCR detection.